To provide broad access to mature technologies, developed within the NRTDP, we have designed a set of specialized service lines offered under the auspices of the Northwestern Proteomics Core Facility. As institutional and regional proteomics cores are well equipped to provide traditional protein identification services, our service lines will represent core competencies of the NRTDP that may not be widely available. The service lines enumerated below will be developed with Standard Operating Procedures and will function robustly.
Characterization of Proteins with Top-Down Mass Spectrometry
Top Down Mass Spectrometry is a technique to characterize the entire sequence of a protein with its related modifications. This service line is tailored to the comprehensive analysis of semi-pure proteins with minimal non-target protein contamination.
Epiproteomic Histone Modification Panel B
The Epiproteomic Histone Modification Panel B (EHMP) provides rapid, low-cost profiling of histone modification states within a sample. While not providing the depth and localization of ChIPSeq analysis, the EHMP allows researchers to generate hypotheses for future study. At present, these 48 modification states are assayed.
Quantitative Translational Top Down Proteomics
Quantitative top-down proteomics is a new service line offered by the NRTDP. Contact us to learn more about this technique.
Peptide Quantitation (SILAC/iTraq/TMT/Label-Free)
Quantitation always requires a consultation to help design the experiment. Depending on the problem, we can use a variety of methods to perform the analysis.
Top-Down Antibody Profiling
Antibody analysis and profiling is a hot topic within the field of proteomics. With our Orbitrap-based mass spectrometers and custom methods, we can provide relative quantitation of antibodies and tandem MS information to confirm its sequence.
If you are interested in collaborating with the NRTDP, please fill out a Collaboration Request.
Acknowledging the Resource
Please acknowledge work performed within the NRTDP with text similar to this: This research was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health and supported by the Sherman Fairchild Foundation.
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