Identification of proteoform biomarkers in liver transplant rejection

In collaboration with:

 

Description: Acute rejection after liver transplantation is the leading cause of mortality among liver transplanted recipients (LTRs). Unfortunately, transplantation prognosis is based on invasive procedures like biopsy. We are thus applying mass spectrometry-based top-down proteomics to detect differentially expressed proteoforms (DEPs) from LTR’s blood samples. Ultimately, our goal is to develop a minimally invasive, fast, and sensitive assay for diagnostic purposes. Previously, we performed a top-down quantitative analysis of peripheral blood mononuclear cells (PBMCs) from 75 LTRs and determined a set of proteoforms to develop a targeted quantitative assay named proteoform reaction monitoring (PfRM). We are now improving our PfRM assay to be more selective and sensitive.

 

 

Project status: On going

Publications:

-Proteoforms in Peripheral Blood Mononuclear Cells as Novel Rejection Biomarkers in Liver Transplant Recipients. 2017. DOI: 10.1111/ajt.14359

-A comprehensive pipeline for translational top-down proteomics from a single blood draw. 2019. DOI: 10.1038/s41596-018-0085-7

-BPA (only after January 28)

Congress Presentations:

– ASMS 2019, ASMS 2020, ASMS 2021

Spinoff funding: R21 AI135827-02 – 2018 to 2021